COLORIMETRIC METHODS FOR DETERMINING SERUM CHOLESTEROL
نویسندگان
چکیده
منابع مشابه
Endpoint colorimetric method for assaying total cholesterol in serum with cholesterol dehydrogenase.
BACKGROUND Various methods are available to measure serum cholesterol concentrations. Of these, the cholesterol ester hydrolase (CEH)-cholesterol oxidase-peroxidase chromogenic method is widely used. However, this method has the disadvantage of interference by reducing substances. We developed and evaluated an endpoint assay for serum cholesterol, based on a CEH-cholesterol dehydrogenase (CDH)-...
متن کاملThe colorimetric estimation of free and combined cholesterol in serum.
This paper describes a simple and quick method for determination of free and com bined cholesterol in, serum. This method requires less hours to complete the determina tion as compared to the older method which requires at least twenty-hours. The total choleterol is first determined by Zuckerman's method(1). Combined and free cholesterol are determined on the chloroform extract with the aid of ...
متن کاملEvaluation of alternative calculation methods for determining LDL cholesterol.
BACKGROUND Due to limitations of the Friedewald formula, alternative methods for calculating low-density lipoprotein cholesterol (LDL-C) were suggested. We evaluated utility of these methods. METHODS Ninety three subjects free of coronary heart disease were considered. LDL-C was measured by the homogeneous method, and calculated by the Friedewald formula LDL-C = TC-HDL-(TG/2.2) (LDL1) and alt...
متن کاملFive methods for determining low-density lipoprotein cholesterol compared.
We evaluated three precipitation methods for determination of low-density lipoprotein cholesterol in serum and an indirect method involving the Friedewald formula (Clin Chem 18: 499-502, 1972) by comparison with results by ultracentrifugation. The results of all methods for 83 sera, including 59 hyperlipidemic type IIA, IIB, and IV sera agreed very well, at least for concentrations of serum tri...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1917
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)86823-5